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phosphopeptides  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology phosphopeptides
    Phosphopeptides, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphocruz+protein+purification+system/PhosphoCruz+Protein+Purification+System/pm32804243-108-15-20
    Average 93 stars, based on 16 article reviews
    phosphopeptides - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Protein Purification:

    Article Title: Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
    Article Snippet: Monoclonal mouse immunoglobulin (Ig) G1 antibodies to human HSP 70 (catalog number 610607) and 90 (catalog number 610418) were purchased from BD Biosciences (San Jose, California). .. Phosphocruz protein purification system (sc-24964) and Cruz Marker molecular weight standards (sc-2035) were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, California). .. Mouse antiphosphotyrosine recombinant clone 4G10 (catalog number 05- 10506), mouse antiphosphoserine (catalog number 05-1000), rabbit antiphosphothreonine (catalog number AB-1607), normal mouse IgG, and Amicon Ultra-4 10,000-kDa nominal molecular weight limit tubes were purchased from Millipore (Billerica, Massachusetts; Temecula, California).

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. Immobilized Metal-Affinity Chromatography ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Article Title: Ca 2+ /Calmodulin-Dependent Protein Kinase II Is a Modulator of CARMA1-Mediated NF-?B Activation
    Article Snippet: .. Phosphoproteins were isolated via IMAC using a PhosphoCruz protein purification system (Santa Cruz Biotechnology, CA) according to the manufacturer's instructions. .. Briefly, 25 μl of sample lysates was diluted with 1 ml of binding/washing buffer (Santa Cruz Biotechnology) and reacted with 10 μl of PhosphoCruz agarose for 1 h at 4°C.

    Article Title: Ca 2+ /Calmodulin-Dependent Protein Kinase II Is a Modulator of CARMA1-Mediated NF-κB Activation
    Article Snippet: .. Phosphoproteins were isolated via IMAC using a PhosphoCruz protein purification system (Santa Cruz Biotechnology, CA) according to the manufacturer’s instructions. .. Briefly, 25 l of sample lysates was diluted with 1 ml of binding/washing buffer (Santa Cruz Biotechnology) and reacted with 10 l of PhosphoCruz agarose for 1 h at 4°C.

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Article Title: Phosphorylation of HOX11/TLX1 on Threonine-247 during mitosis modulates expression of cyclin B1
    Article Snippet: .. Phosphoenriched lysates following nocodazole treatment were generated using the PhosphoCruz Protein Purification System (Santa Cruz) according to manufacturer's protocols. .. Staurosporine (Sigma) was added in conjunction with nocodazole at concentrations of 0, 20, 40, 60, 80 and 100 nM.

    Article Title: 4-Methylumbelliferone inhibits the phosphorylation of hyaluronan synthase 2 induced by 12-O-tetradecanoyl-phorbol-13-acetate.
    Article Snippet: .. Phosphorylated and non-phosphorylated proteins in the supernatant were fractionated by PhosphoCruz Protein Purification System, using an immobilized metal affinity chromatography (IMAC) column (Santa Cruz Biotechnology). ..

    Marker:

    Article Title: Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
    Article Snippet: Monoclonal mouse immunoglobulin (Ig) G1 antibodies to human HSP 70 (catalog number 610607) and 90 (catalog number 610418) were purchased from BD Biosciences (San Jose, California). .. Phosphocruz protein purification system (sc-24964) and Cruz Marker molecular weight standards (sc-2035) were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, California). .. Mouse antiphosphotyrosine recombinant clone 4G10 (catalog number 05- 10506), mouse antiphosphoserine (catalog number 05-1000), rabbit antiphosphothreonine (catalog number AB-1607), normal mouse IgG, and Amicon Ultra-4 10,000-kDa nominal molecular weight limit tubes were purchased from Millipore (Billerica, Massachusetts; Temecula, California).

    Molecular Weight:

    Article Title: Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
    Article Snippet: Monoclonal mouse immunoglobulin (Ig) G1 antibodies to human HSP 70 (catalog number 610607) and 90 (catalog number 610418) were purchased from BD Biosciences (San Jose, California). .. Phosphocruz protein purification system (sc-24964) and Cruz Marker molecular weight standards (sc-2035) were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, California). .. Mouse antiphosphotyrosine recombinant clone 4G10 (catalog number 05- 10506), mouse antiphosphoserine (catalog number 05-1000), rabbit antiphosphothreonine (catalog number AB-1607), normal mouse IgG, and Amicon Ultra-4 10,000-kDa nominal molecular weight limit tubes were purchased from Millipore (Billerica, Massachusetts; Temecula, California).

    Chromatography:

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. Immobilized Metal-Affinity Chromatography ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Isolation:

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. Immobilized Metal-Affinity Chromatography ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Article Title: Ca 2+ /Calmodulin-Dependent Protein Kinase II Is a Modulator of CARMA1-Mediated NF-?B Activation
    Article Snippet: .. Phosphoproteins were isolated via IMAC using a PhosphoCruz protein purification system (Santa Cruz Biotechnology, CA) according to the manufacturer's instructions. .. Briefly, 25 μl of sample lysates was diluted with 1 ml of binding/washing buffer (Santa Cruz Biotechnology) and reacted with 10 μl of PhosphoCruz agarose for 1 h at 4°C.

    Article Title: Ca 2+ /Calmodulin-Dependent Protein Kinase II Is a Modulator of CARMA1-Mediated NF-κB Activation
    Article Snippet: .. Phosphoproteins were isolated via IMAC using a PhosphoCruz protein purification system (Santa Cruz Biotechnology, CA) according to the manufacturer’s instructions. .. Briefly, 25 l of sample lysates was diluted with 1 ml of binding/washing buffer (Santa Cruz Biotechnology) and reacted with 10 l of PhosphoCruz agarose for 1 h at 4°C.

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Affinity Chromatography:

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. Immobilized Metal-Affinity Chromatography ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Article Title: Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis
    Article Snippet: The specimen was observed with an electron microscope (JEM-1200 EX II; JEOL) operated at 80 kV. .. ER vesicles isolated from MM2d cells were solubilized with 1% Triton X-100 and subjected to immobilized metal affinity chromatography according to the manufacturer’s instructions (PhosphoCruz Protein Purification System; Santa Cruz Biotechnology). ..

    Article Title: 4-Methylumbelliferone inhibits the phosphorylation of hyaluronan synthase 2 induced by 12-O-tetradecanoyl-phorbol-13-acetate.
    Article Snippet: .. Phosphorylated and non-phosphorylated proteins in the supernatant were fractionated by PhosphoCruz Protein Purification System, using an immobilized metal affinity chromatography (IMAC) column (Santa Cruz Biotechnology). ..

    other:

    Article Title: P-Rex1 participates in Neuregulin-ErbB signal transduction and its expression correlates with patient outcome in breast cancer.
    Article Snippet: The cytosolic fraction was loaded in a column (PhosphoCruz Protein Purification System, Santa Cruz Biotechnology) that contained a matrix for the purification of phosphorylated proteins.

    Generated:

    Article Title: Phosphorylation of HOX11/TLX1 on Threonine-247 during mitosis modulates expression of cyclin B1
    Article Snippet: .. Phosphoenriched lysates following nocodazole treatment were generated using the PhosphoCruz Protein Purification System (Santa Cruz) according to manufacturer's protocols. .. Staurosporine (Sigma) was added in conjunction with nocodazole at concentrations of 0, 20, 40, 60, 80 and 100 nM.



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